Spectrophotometric determination of leukocytes in urine
A spectrophotometric method based on myeloperoxidase activity for the determination of leukocytes in urine is described. Red cells that may be found in urine samples were lysed by an ammonium chloride method. Leukocytes were then sedimented by centrifugation and lysed using Triton X-100 (Sigma Chemicals Co., St. Louis, MO).
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